a study on the genetic analysis of clinical isolates and vaccine strains of bordetella pertussis by pulsed-field gel electrophoresis (pfge)

نویسندگان

a. bahmanjeh

department of microbiology, faculty of veterinnary, islamic azad university of karaj, karaj, iran p. khaki

razi vaccine and serum research institute, agricultural research, education and extension organization, tehran, iran s. moradi bidhendi

razi vaccine and serum research institute, agricultural research, education and extension organization, tehran, iran r. hosseinpour

razi vaccine and serum research institute, agricultural research, education and extension organization, tehran, iran m. noofeli

چکیده

considering the circulation of bordetella pertussis clinical strains among populations with high vaccination coverage, it is necessary to have a proper understanding of this bacterium causing whooping cough. various techniques, which are available for studying b. pertussis, can facilitate a proper comparison between different populations. we genotypically analyzed a collection of two vaccine strains used for the production of killed pertussis vaccine during 2000-2014 at razi vaccine and serum research institute, karaj, iran. ten clinical and two reference (tohama 1 and 18323) strains were used by means of pulsed-field gel electrophoresis (pfge). the genetic profiles of the vaccine master and working seeds showed no significant changes in the frequency of fingerprint types in the vaccine strains; also, the homogeneity of the profiles was demonstrated. however, the clinical isolates showed heterogeneity in the genetic profiles. in addition, serotyping was performed with monoclonal antisera to agglutinogens 2 and 3. analysis of the fimbriae showed that all ten clinical strains expressed fim3.

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منابع مشابه

A study on the genetic analysis of clinical isolates and vaccine strains of Bordetella pertussis by pulsed-field gel electrophoresis (PFGE)

Considering the circulation of Bordetella pertussis clinical strains among populations with high vaccination coverage, it is necessary to have a proper understanding of this bacterium causing whooping cough. Various techniques, which are available for studying B. pertussis, can facilitate a proper comparison between different populations. We genotypically analyzed a collection of two vaccine st...

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archives of razi institute

جلد ۷۱، شماره ۴، صفحات ۲۱۹-۲۲۵

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